Table A-1. Limiting Conditions for Pathogen Growth (FDA, 2001). 
Updated: 06/08/07
Pathogen min. aw
(using salt)
min. pH max. pH max. % water
phase salt
min. temp. max. temp. oxygen requirement
Bacillus cereus .92 4.3 9.3 10 39.2°F
4°C
131°F4
55°C
aerobe
Campylobacter jejuni .987 4.9 9.5 1.5 86°F
30°C
113°F
45°C
microaerophilic1
Clostridium botulinum, type A, and proteolytic B and F .935 4.6 9 10 50°F
10°C
118.4°F
48°C
anaerobe2
Clostridium botulinum, type E, and nonproteolytic B and F .97 5 9 5 37.9°F
3.3°C
113°F
45°C
anaerobe2
Clostridium perfringens .93 5 9 7 50°F
10°C
125.6°F
52°C
anaerobe2
pathogenic strains of Escherichia coli .95 4 9 6.5 43.7°F
6.5°C
120.9°F
49.4°C
facultative anaerobe3
Listeria monocytogenes .92 4.4 9.4 10 31.3°F
-0.4°C
113°F
45.4°C
facultative anaerobe3
Salmonella spp. .94 3.7 9.5 8 41.4°F
5.2°C
115.2°F
46.2°C
facultative anaerobe3
Shigella spp. .96 4.8 9.3 5.2 43°F
6.1°C
116.8°F
47.1°C
facultative anaerobe3
Staphylococcus aureus
growth
Staphylococcus aureus
toxin
.83

.85
4

4
10

9.8
20

10
44.6°F
7°C
50°F
10°C
122°F
50°C
118°F
48°C
facultative anaerobe3
Vibrio cholerae .97 5 10 6 50°F
10°C
109.4°F
43°C
facultative anaerobe3
Vibrio parahaemolyticus .94 4.8 11 10 41°F
5°C
113.5°F
45.3°C
facultative anaerobe3
Vibrio vulnificus .96 5 10 5 46.4°F
8°C
109.4°F
43°C
facultative anaerobe3
Yersinia enterocolitica .945 4.2 10 7 29.7°F
-1.3°C
107.6°F
42°C
facultative anaerobe3
1requires limited levels of oxygen
2
requires the absence of oxygen
3
grows either with or without oxygen
4
growth significantly delayed (>24 hr.) at 131°F (55°C)

References

FDA. 2001. Table #A-1: Limiting Conditions for Pathogen Growth. Appendix 4. In Fish and Fishery Products Hazards and Controls Guide, 3rd ed., p. 281. Department of Health and Human Services, Public Health Service, Food and Drug Administration, Center for Food Safety and Applied Nutrition, Office of Seafood, Washington, DC.

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